Part 40 fundamentals
Collector responsibilities, privacy, specimen integrity and applicable DOT procedures.
DOT collector qualification training
DOT Specimen Collector Training
Learn the DOT urine collection process, Federal Custody and Control Form procedures, specimen integrity safeguards and the requirements for successful mock collection demonstrations. Build the practical knowledge required under 49 CFR Part 40.
49 CFR Part 40DOT collector procedures
Five mock collectionsRequired error-free demonstrations
Federal CCF trainingCollection records and corrections
Online learningIndividual and trainer options
What you will learn
Training for each step of a compliant collection.
From site preparation to completing the Federal CCF, explore the key responsibilities of a DOT urine specimen collector.
Prepare the collection site
Site security, collection supplies, donor privacy and measures to prevent adulteration.
Federal CCF
Form completion, donor and collector signatures, seals, copy distribution and corrections.
Urine collection procedure
Specimen provision, temperature checks, split specimens, sealing and shipment.
Problem collections
Insufficient specimen, out-of-range temperature, directly observed collections and refusals.
Qualification demonstrations
Practice scenarios and documentation for the required error-free mock collections.
Practical proficiency
Online lessons are only part of collector qualification.
DOT collector qualification under 49 CFR §40.33 also requires five consecutive error-free mock collections with a qualified monitor. Review the required scenarios and the next steps for documenting proficiency.
Compare Qualification Plans01Routine collectionDemonstrate a standard collection without procedural errors.
02Insufficient specimenApply the required shy-bladder procedure.
03Temperature outside rangeRespond correctly when the specimen temperature is outside the accepted range.
04Refusal to sign or initialHandle a donor refusing a required signature or initials.
05Second routine collectionDemonstrate the required second uneventful collection and document each mock collection.
Course enrollment
Choose your collector training plan.
Current prices are loaded from the training catalog. Select the plan that matches your role and course requirements.
Collector Training
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Build foundational DOT urine collector skills.
- Part 40 collector curriculum
- Mock collection qualification pathway
- 60-day course access
- Completion documentation pathway
Collector + Hair
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Collector training with hair collection instruction.
- DOT urine collector curriculum
- Hair collection procedures
- Hair collection completion pathway
- 60-day course access
Train the Trainer
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Collector curriculum with trainer resources.
- Full collector curriculum
- Instructor and trainer materials
- Instructor completion pathway
- 60-day course access
Trainer + Hair
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Trainer curriculum with hair collection instruction.
- Train the Trainer content
- Hair collection procedures
- Instructor and hair course pathways
- 60-day course access
Who should enroll
Training for new and experienced collectors.
New collectors
- Professionals beginning DOT urine specimen collection work
- Collection-site staff learning Part 40 procedures
- Employer personnel assigned to conduct collections
Experienced collectors
- Collectors seeking requalification or refresher instruction
- Professionals needing error-correction retraining
- Teams standardizing collection-site procedures
DOT regulations
Part 40 procedures support multiple DOT operating modes.
DOT drug and alcohol testing procedures are supplemented by the rules applicable to each regulated industry.
Motor carriers
49 CFR Part 382 for covered CDL drivers and employers.
Aviation
14 CFR Part 120 for covered aviation employers and personnel.
Railroads
49 CFR Part 219 for covered railroad operations.
Transit
49 CFR Part 655 for covered transit systems.
Pipeline
49 CFR Part 199 for covered pipeline employees.
Maritime
46 CFR Parts 4 and 16 for covered maritime operations.
Frequently asked questions
Collector training questions, answered.
Does the online course alone qualify me as a DOT collector?
No. Qualification also requires the proficiency demonstrations specified under 49 CFR §40.33, including five consecutive error-free mock collections. Maintain documentation of both training and proficiency.
How many mock collections are required?
DOT requires five consecutive error-free mock collections, including routine and problem collection scenarios, monitored by a qualified person.
Can someone new to collections enroll?
Yes. The collector course is intended for people learning Part 40 collection procedures as well as existing collectors reviewing those requirements.
How long do I have access?
The collector plans listed here provide 60 days of course access. Confirm the exact terms for your selected plan during enrollment.
Can my employer enroll several people?
Yes. Explore the Group Training page for multiple learners. Each collector must complete their own qualification requirements.
Where do I find my course after purchase?
Use Student Sign In to access assigned training. For more time, review Course Extensions.
Ready to get started?
Train with confidence. Collect with care.
Choose an individual collector course or explore training for your team.