DOT testing overview
Part 40, agency rules, covered employees, testing categories, and employee responsibilities.
Give safety-sensitive employees clear, role-appropriate education on prohibited drug and alcohol conduct, testing circumstances, the testing experience, violations, and the employer’s DOT program expectations. Online DOT employee drug and alcohol awareness training helps safety-sensitive workers understand program expectations.

Course content is structured around current U.S. Department of Transportation rules, agency-specific requirements, and practical workplace responsibilities.
Part 40, agency rules, covered employees, testing categories, and employee responsibilities.
Effects and consequences of prohibited drug use on health, safety, and the work environment.
Prohibited alcohol conduct, testing thresholds, timing restrictions, and workplace safety.
What happens during collections/tests, refusals, problem specimens, and result reporting.
Removal from safety-sensitive work, SAP evaluation, education/treatment, return-to-duty and follow-up testing.
How the employer’s policy, contacts, assistance resources, and disciplinary rules fit into the DOT framework.
Individual and volume options make it easier to train a regulated workforce consistently.
For one safety-sensitive employee.
For small safety-sensitive teams.
For growing safety-sensitive workforces.
For larger workforces.
49 CFR Part 40 provides common DOT drug and alcohol testing procedures. Covered employers also operate under their modal agency rule.
Drug and alcohol program requirements for covered aviation employers and safety-sensitive employees.
Drug and alcohol testing requirements for CDL drivers and regulated motor carriers.
Drug and alcohol requirements for regulated railroad employees and employers.
Drug and alcohol program requirements for covered public transportation employers and employees.
Drug and alcohol requirements for pipeline operators and covered employees.
Maritime drug and alcohol program requirements for covered vessel operations and personnel.
Find information about DOT employee education, testing rules, enrollment, and course access.
This course is intended for employees performing safety-sensitive duties under DOT-regulated drug and alcohol testing programs. Employers should assign education that matches the applicable agency rule and each employee's responsibilities.
The curriculum addresses prohibited drug and alcohol conduct, circumstances that can require testing, employee responsibilities, the testing process, and the consequences of violations under applicable DOT rules.
Employee education and training requirements differ among DOT operating administrations. Employers must confirm which instruction, materials, and training duration apply to their workforce rather than assuming one course satisfies every agency's requirements.
Depending on the applicable DOT program, testing may include pre-employment, random, reasonable suspicion or reasonable cause, post-accident, return-to-duty, and follow-up testing. Not every test type applies in the same way to every agency or situation.
A refusal under applicable DOT rules can result in removal from safety-sensitive functions and additional return-to-duty requirements before the employee may resume covered work. Employers must follow the relevant DOT definitions and procedures.
Yes. This page offers individual and multi-employee enrollment plans. You can also visit Group Training to review options for larger teams.
Course completion documentation is provided according to the selected training plan and applicable completion requirements. Employers should retain records needed for their regulated program.
Employees can access assigned courses through the Student Login. For additional access options, visit the Course Extensions page.